卵胞漿內單精子注射技術(intracytoplasmic sperm injection, ICSI)）完全或不完全受精失敗非常罕見，但是部分患者由于精子缺乏激活卵母細胞的物質而導致ICSI反復的完全或不完全受精失敗。本文報道的是一例嚴重的弱畸精子患者，出現了反復的ICSI不完全受精失敗。在患者的第二個ICSI周期中，10枚卵母細胞經ICSI技術受精后，僅有一枚卵母細胞受精。遂采用鈣離子載體A23187對ICSI未受精卵母細胞進行激活處理。未受精卵母細胞激活，獲得胚胎，移植后獲得臨床妊娠，并健康新生兒的出生。由此可見，對ICSI未受精卵母細胞實施“補救性卵母細胞激活技術”能挽救ICSI完全或不完全受精失敗。北京大學人民醫(yī)院計劃生育與生殖醫(yī)學科鹿群

[Abstract]

Total or near-total fertilization failure after intracytoplasmic sperm injection (ICSI) is a rare event, but it occurs repeatedly because of sperm defects in activating oocyte. The case presents a successful pregnancy and live birth after calcium ionophore A23187 (A23187) activation on one-day-old unfertilized oocytes in a patient whose husband suffered oligoasthenoteratozoospermia, and who had experienced repeated near-total fertilization failure after ICSI. In the second ICSI cycle, only one oocyte was fertilized while nine were unfertilized. Oocyte activation with A23187 were performed on the one-day-old unfertilized oocytes after ICSI and resulted in fertilization and embryo transfer. A clinical pregnancy was achieved and a healthy baby was born. To our knowledge, this is the first reported case of a healthy birth after oocyte activation on the one-day-old unfertilized oocyte. This indicates that “rescue oocyte activation” on one-day-old unfertilized oocytes after ICSI may be help for preventing total or near-total fertilization failure after ICSI.

Chin Med J 2012;125(3):546-548